Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bandari, C.; Scull, E.M.; Masterson, J.M.; Tran, R.H.Q.; Foster, S.B.; Nicholas, K.M.; Singh, S.
    Determination of alkyl-donor promiscuity of tyrosine-O-prenyltransferase SirD from Leptosphaeria maculans (2017), ChemBioChem, 18, 2323-2327 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.122 synthesis the broad alkyl donor specificity of SirD opens the door for the interrogation of the alkyl donor specificity of other prenyltransferases for potential utility as biocatalysts for differential alkylation applications Leptosphaeria maculans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.122 expressed in Escherichia coli BL21(DE3) cells Leptosphaeria maculans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.122 0.05
-
 (2Z)-3-chlorobut-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.07
-
 (2E)-but-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.12
-
 (2E,4Z)-2-methylhexa-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.13
-
 cyclohex-1-en-1-ylmethyl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.17
-
 (2E)-2-methylpenta-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.2
-
 (2E)-3-methylpent-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.21
-
 2-cyclohexylideneethyl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.23
-
 dimethylallyl diphosphate with L-tyrosine as cosubstrate, at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.24
-
 3-ethylpent-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.26
-
 (2E)-penta-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.73
-
 (2E)-5-methylhexa-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.122 Mg2+ 5 mM used in assay conditions Leptosphaeria maculans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.122 dimethylallyl diphosphate + L-tyrosine Leptosphaeria maculans
-
 diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.122 Leptosphaeria maculans Q6Q874
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.122 Ni-NTA column chromatography Leptosphaeria maculans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.122 (2E)-2-methylpenta-2,4-dien-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[(2E)-2-methylpenta-2,4-dien-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 (2E)-3-(2-methoxyphenyl)prop-2-en-1-yl trihydrogen diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[(2E)-3-(2-methoxyphenyl)prop-2-en-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 (2E)-3-methylpent-2-en-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[(2E)-3-methylpent-2-en-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 (2E)-3-phenylbut-2-en-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[(2E)-3-phenylbut-2-en-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 (2E)-3-phenylprop-2-en-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[(2E)-3-phenylprop-2-en-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 (2E)-5-methylhexa-2,4-dien-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[(2E)-5-methylhexa-2,4-dien-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 (2E)-but-2-en-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[(2E)-but-2-en-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 (2E)-penta-2,4-dien-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[(2E)-penta-2,4-dien-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 (2E,4E)-2-methylhexa-2,4-dien-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[(2E,4E)-2-methylhexa-2,4-dien-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 (2E,4E)-5-phenylpenta-2,4-dien-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[(2E,4E)-5-phenylpenta-2,4-dien-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 (2Z)-3-chlorobut-2-en-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[(2Z)-3-chlorobut-2-en-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 2-cyclohexylideneethyl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[2-cyclohexylideneethyl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 2-methylprop-2-en-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[2-methylprop-2-en-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 2-oxopropyl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[2-oxopropyl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 3,3-diphenylprop-2-en-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[3,3-diphenylprop-2-en-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 3-ethylpent-2-en-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[3-ethylpent-2-en-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 cyanomethyl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[cyanomethyl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 cyclohex-1-en-1-ylmethyl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[cyclohex-1-en-1-ylmethyl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 dimethylallyl diphosphate + L-tryptophan
-
 Leptosphaeria maculans diphosphate + 7-dimethylallyl-L-tryptophan
-
 ?
2.5.1.122 dimethylallyl diphosphate + L-tyrosine
-
 Leptosphaeria maculans diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?
2.5.1.122 prop-2-en-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[prop-2-en-1-yl]-L-tyrosine + diphosphate
-
 ?
2.5.1.122 prop-2-yn-1-yl diphosphate + L-tyrosine
-
 Leptosphaeria maculans O-[prop-2-yn-1-yl]-L-tyrosine + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.122 SirD
-
 Leptosphaeria maculans
2.5.1.122 tyrosine O-prenyltransferase
-
 Leptosphaeria maculans
2.5.1.122 tyrosine-O-prenyltransferase
-
 Leptosphaeria maculans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.122 0.00067
-
 (2Z)-3-chlorobut-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.00083
-
 (2E)-penta-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.001
-
 (2E)-but-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.00167
-
 (2E)-5-methylhexa-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.00367
-
 (2E)-2-methylpenta-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.0055
-
 cyclohex-1-en-1-ylmethyl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.0123
-
 (2E,4Z)-2-methylhexa-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.055
-
 3-ethylpent-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.0583
-
 2-cyclohexylideneethyl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.3
-
 (2E)-3-methylpent-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.683
-
 dimethylallyl diphosphate with L-tyrosine as cosubstrate, at pH 7.5 and 35°C Leptosphaeria maculans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.122 0.0023
-
 (2E)-5-methylhexa-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.0033
-
 (2E)-penta-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.015
-
 (2E)-but-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.0167
-
 (2Z)-3-chlorobut-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.0217
-
 (2E)-2-methylpenta-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.042
-
 cyclohex-1-en-1-ylmethyl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.1017
-
 (2E,4Z)-2-methylhexa-2,4-dien-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.23
-
 3-ethylpent-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 0.267
-
 2-cyclohexylideneethyl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 1.5
-
 (2E)-3-methylpent-2-en-1-yl diphosphate at pH 7.5 and 35°C Leptosphaeria maculans
2.5.1.122 2.97
-
 dimethylallyl diphosphate with L-tyrosine as cosubstrate, at pH 7.5 and 35°C Leptosphaeria maculans